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You are here:Home » Antibodies » Abs to Clathrin » Anti -Clathrin

Anti -Clathrin

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH
Catalog #C5835-04
ApplicationsSuitable for use in Immunohistochemistry (frozen tissue) and Immunoblot (Western).
Recommended DilutionImmunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1 h at RT.
Immunoblot: 1:100-1:500 (ECL).
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgM
Clone No5F178
HostMouse
SourceBovine
Concentration~0.1mg/ml
FormSupplied as a lyophilized powder. Reconstitute to 1ml final volume.
PurityGel filtration.
ImmunogenCoated vesicles (clathrin) of bovine brain.
SpecificitySpecies Crossreactivity: Human, bovine, porcine, rat, amphibia. Reactivities on Cultured HeLa, SV-40, RVF-SMC Cell Lines (tested so far). Specifically Detected organs and tissues (eg brain, mammary gland, ovaries). This clone represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e.g. refs. 2 and 3). Polypeptide Reacting Mr 180,000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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