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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B IH

Catalog #	C5835-04
Applications	Suitable for use in Immunohistochemistry (frozen tissue) and Immunoblot (Western).
Recommended Dilution	Immunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1 h at RT.
	Immunoblot: 1:100-1:500 (ECL).
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgM
Clone No	5F178
Host	Mouse
Source	Bovine
Concentration	~0.1mg/ml
Form	Supplied as a lyophilized powder. Reconstitute to 1ml final volume.
Purity	Gel filtration.
Immunogen	Coated vesicles (clathrin) of bovine brain.
Specificity	Species Crossreactivity: Human, bovine, porcine, rat, amphibia. Reactivities on Cultured HeLa, SV-40, RVF-SMC Cell Lines (tested so far). Specifically Detected organs and tissues (eg brain, mammary gland, ovaries). This clone represents an excellent marker for detection of receptor mediated endocytosis (for review cf. e.g. refs. 2 and 3). Polypeptide Reacting Mr 180,000 polypeptide (clathrin heavy chain) from coated vesicles of various tissues.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.