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You are here:Home » Antibodies » Abs to Clathrin » Anti -Clathrin

Anti -Clathrin

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum B IH
Catalog #C5835-05A
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. SUBUNIT Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium.
Cellular LocalizationCytoplasmic vesicle; cytoplasmic vesicle membrane; cytoplasmic side. Cell membrane; coated pit; cytoplasmic side. Melanosome. Note=Cytoplasmic face of coated pits and vesicles. PTM: The N-terminus is blocked. Belongs to the clathrin heavy chain family.
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
PuritySerum
ImmunogenA synthetic peptide (KAGLLQRALEHFTDLYDIKR) corresponding to the amino acids 619-638 of rat clathrin conjugated to diphtheria toxin has been used as the immunogen. The peptide is homologous with the corresponding sequence derived from clathrin protein in mouse, human and bovine.
SpecificityThis antibody has been shown to be specific for clathrin. Species Crossreactivity: Rat, other species have not yet tested.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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