Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Clathrin » Anti -Clathrin, Heavy Chain

Anti -Clathrin, Heavy Chain

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IC IF
Clathrin-coated pits, intracellular transport vesicles involved in endocytosis and other membrane trafficking processes, are multi-component units. (1,2). Clathrin, the most abundant protein in these vesicles, is formed of a basic assembly unit called a triskelion composed of three clathrin heavy chains and three clathrin light chains. Together with other coat components such as AP2, epsin and EPS15, triskelia are targeted to appropriate membrane fusion sites, where subsequent invagination, cargo protein uptake and vesicle internalization occurs (3,4).
Catalog #C5835-07Y
ApplicationsSuitable for use in Western Blot, Immunofluorescence/ Immunocytochemistry. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Immunofluorescence / Immunocytochemistry: 1:400
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to residues surrounding Pro1663 of human Clathrin conjugated to KLH. Species sequence homology: porcine
SpecificityRecognizes endogenous levels of total human Clathrin protein at 190kD. Species Crossreactivity: mouse, rat, monkey, bovine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links