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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IC IF

Clathrin-coated pits, intracellular transport vesicles involved in endocytosis and other membrane trafficking processes, are multi-component units. (1,2). Clathrin, the most abundant protein in these vesicles, is formed of a basic assembly unit called a triskelion composed of three clathrin heavy chains and three clathrin light chains. Together with other coat components such as AP2, epsin and EPS15, triskelia are targeted to appropriate membrane fusion sites, where subsequent invagination, cargo protein uptake and vesicle internalization occurs (3,4).
Catalog #	C5835-07Y
Applications	Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Immunofluorescence (IF-IC): 1:400
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide (KLH-coupled) corresponding to residues surrounding Pro1663 of human Clathrin.
Specificity	Detects endogenous levels of total Clathrin protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.