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You are here:Home » Antibodies » Abs to Clathrin » Anti -Clathrin, Light Chain (BSA & Azide Free)

Anti -Clathrin, Light Chain (BSA & Azide Free)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP IH IF
Clathrin consists of three heavy chain (192kD) and three light chains (LCa or LCb, 25-29kD). It self-assembles into a polyhedral cytoskeletal element. Self-assembly facilitates clathrin’s central role in membrane sorting and selective transport within the cell.
Catalog #C5835-08X
ApplicationsSuitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Denatured verified): 2ug/mg protein using Protein A.
Western Blot: 1-2ug/ml for 2hrs at RT
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control Placenta
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No3F133
HostMouse
SourceBovine
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C5835-08.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide derived from bovine clathrin light chain corresponding to the conserved region of the light chains.
MW of Antigen25-29kD Epitope: aa23-44
Cellular LocalizationCell membrane and cytoplasm
SpecificityRecognizes both bovine LCa and LCb. Species Crossreactivity: human
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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