| Chloride is a critical component of all living cells. Voltage-gated chloride channels regulate cellular traffic of chloride ion. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease, and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000 aa) are predicted to contain 10 (possibly 12) transmembrane domains.Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues. |
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| Catalog # | C5837-21 |
| Rat CLC-4 is 747 aa membrane protein (human CLC-4 760; mouse CLC-4 747 aa) (1). CLC-4 is abundant in liver, brain moderate levels are found in the heart, muscle, kidney, and spleen. |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot:1-10ug/ml using ECL. |
| ELISA: 0.5-1ug/ml for affinity purified. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Host | Rabbit |
| Source | Rat |
| Concentration | 1mg/ml |
| Form | Supplied as a liquid in Tris, pH 7.5 and 0.2% BSA, 0.05% sodium azide, 40% glycerol. |
| Purity | Purified over the peptide-Sepharose column |
| Immunogen | A 13 AA Peptide sequence near the C-terminus of rat CLC-4 (1). The peptide was coupled to KLH. |
| Specificity | The 13 AA CLC41-P control peptide is 100% conserved in mouse, rat and human CLC-4. No significant sequence homology is detected with other CLCs or other proteins. There is a very high degree of protein homology among CLC-3, CLC-4 and CLC-5. However, CLC41-P peptide has very little sequence homology with CLC-3 and CLC-5. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
