| Chloride is a critical component of all living cells. Voltage-gated chloride channels regulate cellular traffic of chloride ion. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease, and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000 aa) are predicted to contain 10 (possibly 12) transmembrane domains.Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues. Rat CLC-5 is 746 aa membrane protein (human CLC-5 746) (1). CLC-5 is abundant in kidney moderate levels are found in the brain, lung, and liver. Mutations or deletions in CLC-5 have been linked with Dent's diseases (excessive urinary calcium, urinary low mol wt proteinuria, and calcium kidney stone), and four related X-linked syndromes of hypercalciuric nephrolithiasis. |
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| Applications: |
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| Suitable for use in ELISA and Western Blot. Other applications not tested. |
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| Catalog # | C5837-31 |
| Recommended Dilution | ELISA: 0.5-1ug/ml using the control peptide to coat plates at 1ug/ml. |
| Western Blot: 1-10ug/ml using ECL. Detects a protein of ~80kD in kidney. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Control Peptide | C5837-35: CLC5, Rat (Clcn5, Chloride Channel) Control Peptide |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, 0.1% BSA. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to 13aa from the C-terminus of rat CLC-5 (KLH). |
| Specificity | Recognizes rat CLC5. No significant sequence homology is detected with other CLCs or other proteins. Sequence Homology |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
