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You are here:Home » Antibodies » Abs to Ion Channel » Anti -CLC7 (Clcn7, Chloride Channel)

Anti -CLC7 (Clcn7, Chloride Channel)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Chloride is a critical component of all living cells. Voltage-gated chloride channels regulate cellular traffic of chloride ion. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000aa) are predicted to contain 10 (possibly 12) transmembrane domains. CLC-1 and CLC-K1/K2 that are specific for kidney. Most other CLC are widely distributed in various tissues. Rat CLC-7 is 802aa membrane protein (mouse 803aa, human 805aa) (1). CLC-7 has a calculated MW of ~90kD. CLC-7 is ~45% identical with CLC-6. CLC-7 is expressed in brain, testes, muscle and kidney (1).
Catalog #C5837-52
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1-10ug/ml (ECL). CLC7 is ~80kD (2).
ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Control PeptideC5837-54: CLC7, Rat (Clcn7, Chloride Channel)
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic peptide 23aa sequence near the C-terminus of rat CLC-7 (KLH coupled).
SpecificityRecognizes CLC7. No significant sequence homology is detected with other CLCs or other proteins. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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