| Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell. TJ strands on adjacent cells interact with each other to form a "molecular gasket" that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. In addition to this "barrier" function, the "fence" function of tight junctions plays an important role in maintaining epithelial cell-polarity by blocking the diffusion of membrane proteins between apical (luminal) and basolateral cell surfaces. |
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| Catalog # | C5838-02B |
| Applications | Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | ELISA (Immunogen): 0.1-1ug/ml |
| Western Blot (Canine, Human): 1-2ug/ml |
| Immunofluorescence (Rat): 5-10ug/ml |
| Immunohistochemistry (Human): 5-10ug/ml; For best results in IHC with formalin-fixed, paraffin-embedded tissues, heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.25mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide corresponding to the C-terminus of the human Claudin-1 protein. |
| Specificity | Recognizes the ~22kD human Claudin-1 protein, and does not crossreact with Claudin-3. Reactivity has been confirmed with human Caco-2 and canine MDCK cell lysates and rat kidney homogenates by Western Blot and immunofluorescence, and with human skin tissue by immunohistochemistry. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
