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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified E B IH

Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.
Catalog #	C5838-02C
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry.
	Other applications not tested.
Recommended Dilutions	Western Blot: 2-4ug/ml for 2 hrs at RT
	Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 20 minutes. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
	Optimal dilutions to be determined by the researcher.
Positive Control	T47D cells, Skin or Breast carcinoma.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~0.2mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.
Purity	Purified
Immunogen	Synthetic peptide corresponding to the C-terminus of human Claudin 1. Cellular Localization: Cell membrane on skin and cytoplasmic and nuclear on breast carcinoma.
Specificity	Recognizes human Claudin 1. Species Crossreactivity: monkey, mouse, canine, and rat.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.