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You are here:Home » Antibodies » Abs to Claudin » Anti -Claudin 2 (CLDN2)

Anti -Claudin 2 (CLDN2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IF
Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell. TJ strands on adjacent cells are presumed to interact with each other to form a sort of "molecular gasket" that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. In addition to this so-called "barrier" function, the "fence" function of tight junctions plays an important role in maintaining epithelial cell-polarity by blocking the diffusion of membrane proteins between apical (luminal) and basolateral cell surfaces. Confinement of, for example, the glucose symport to apical surfaces allows glucose to be transported vectorially from the lumen, through the cell, and into the bloodstream. Several peripheral membrane proteins are associated with tight junctions including ZO-1, ZO-2, ZO-3 (members of membrane-associated guanylate-kinase family), cingulin, the 7H6 antigen, Rab-3b, symplekin. While their precise functions are not known, roles for these proteins have been suggested in tight junction assembly and maintenance; signal transduction; and the regulation of tight junction permeability.
Catalog #C5838-16A
ApplicationsSuitable for use in ELISA, Western Blot and Immunofluorescence. Other applications have not been tested.
Recommended DilutionsELISA (Immunogen): 0.1-1ug/ml
Western Blot (Canine, human and rat): 0.5-2ug/ml
Immunofluorescence (Rat): 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by epitope affinity chromatography.
ImmunogenSynthetic peptide derived from the C-terminus of the human Claudin-2 protein.
SpecificityRecognizes rat Claudin 2 protein at ~22kD and does not appear to cross-react with related endogenous proteins. Reactivity was confirmed by Western Blot with rat kidney and by immunostaining of rat small intestine. Species Crossreactivity: human and canine
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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