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You are here:Home » Antibodies » Abs to Claudin » Anti -Claudin 2 (CLDN2)

Anti -Claudin 2 (CLDN2)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E IH
Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition
Catalog #C5838-18
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistochemistry (Formalin/paraffin): 1:100 30 minutes. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.
Positive controlNormal colon and colon carcinoma.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 0.2% BSA, 5mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide derived from the C-terminus of mouse Claudin 2. Cellular Localization
SpecificityRecognizes mouse Claudin 2. Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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