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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IH

Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. TJ strands on adjacent cells are presumed to interact with each other to form a “molecular gasket” that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. The 20 known claudin proteins are major structural components of tight junctions.
Catalog #	C5838-25
Applications	Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 0.1-1.0ug/ml
	Western Blot: 0.5-3ug/ml
	Immunohistochemistry: 0.1-1.2ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A synthetic peptide from the C-terminus of mouse Claudin-3
Specificity	Recognizes mouse Claudin-3 at 22kD.
Species Crossreactivity	Mouse liver and lung tissue extract and canine MDCK and human MCF-7 cell lysates by Western Blot and formalin-fixed, paraffin-embeddedd human normal colon and colon cancer tissues by Immunohistochemistry.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.