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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified IH

Tight junctions (TJ) are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. TJ strands on adjacent cells are presumed to interact with each other to form a sort of “molecular gasket” that prevents ions, water and other molecules from leaking between cells. Claudin-3 is expressed in junctions of the duct epithelia and acinar cells as well as uniformly expressed from the periportal to the pericentral hepatocytes in the liver. Also, Claudin-3 was found to be present or secreted by many of the genes up-regulated in ovarian cancer.
Catalog #	C5838-26
Applications	Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution	Immunohistochemistry: (paraffin-embedded sections). Antigen retrieval by boiling in 10mM sodium citrate (pH 6.0) is needed.
	Optimal dilutions to be determined by the researcher.
Posittive Control	Colon.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	N/A
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	As reported
Form	Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide.
Purity	Purified.
Immunogen	A synthetic peptide from the C-terminus of mouse Claudin 3.
Specificity	Recognizes mouse Claudin 3. Species Crossreactivity: human and canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.