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You are here:Home » Antibodies » Abs to Claudin » Anti -Claudin 3 (CLDN3)

Anti -Claudin 3 (CLDN3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IH
Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.
Catalog #C5838-27
ApplicationsSuitable for use in Immunohistochemistry. Other applications not tested.
Recommended DilutionImmunohistology (Formalin/paraffin): 1:50 for 20 min. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.
Positive Control Small intestine.
Storage and StabilityFor long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.1mg/ml
FormSupplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%.
PurityPurified by immunoaffinity chromatography.
ImmunogenA synthetic peptide derived from the C-terminus of mouse Claudin 3. Cellular Localization: Cell membrane and cytoplasmic.
MW of antigen22-24kD.
SpecificityRecognizes mouse Claudin 3. Species Crossreactivity: Human and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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