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You are here:Home » Antibodies » Abs to Claudin » Anti -Claudin 4 (CLDN4)

Anti -Claudin 4 (CLDN4)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP
Claudin-4 belongs to the 20-member family of claudins, transmembrane proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl– permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines.
Catalog #C5838-35B
ApplicationsSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionELISA (Immunogen): 0.1-1ug/ml
Immunoprecipitation (mouse): 1-3ug/ml
Western Blot (mouse, rat): 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by epitope affinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminal region of the mouse Claudin-4 protein.
SpecificityRecognizes mouse Claudin-4. On Western blots a 22kD band is observed and a 44kD band representing dimerized protein may be observed. Reactivity has been confirmed with mouse and rat kidney homogenates.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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