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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP

Claudin-4 belongs to the 20-member family of claudins, transmembrane proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns, and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl– permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines.
Catalog #	C5838-35B
Applications	Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications have not been tested.
Recommended Dilution	ELISA (Immunogen): 0.1-1ug/ml
	Immunoprecipitation (mouse): 1-3ug/ml
	Western Blot (mouse, rat): 1-3ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by epitope affinity chromatography.
Immunogen	Synthetic peptide corresponding to the C-terminal region of the mouse Claudin-4 protein.
Specificity	Recognizes mouse Claudin-4. On Western blots a 22kD band is observed and a 44kD band representing dimerized protein may be observed. Reactivity has been confirmed with mouse and rat kidney homogenates.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.