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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E FC

Claudin 4 is a multipass membrane protein in the claudin family of tight junction proteins. Claudin 4 is a 209 amino acid molecule with four transmembrane segments. It is preferentially expressed in intestinal mucosa epithelium and is involved in epithelial tissue development. It is also upregulated in several carcinomas. Phosphorylation of claudin 4 regulates paracellular epithelial permeability. Human claudin 4 shares 83% aa sequence identity with mouse and rat claudin 4.
Catalog #	C5838-35C
Applications	Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilutions	Flow Cytometry: 25ug/ml; 10ul labels 1-2.5x10e5 cells in a total reation volume <200ul. Validated for use with PC-3 cells.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	7H177
Host	Mouse
Source	Human
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant protein corresponding to human Claudin 4 expressed in NSO cells (NP_001296). Species Sequence Homology: mouse and rat, 83%
Specificity	Recognizes human Claudin 4. Stains human Claudin 4 transfectants but not irrelevant transfectants.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.