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You are here:Home » Antibodies » Abs to Claudin » Anti -Claudin 5 (CLDN5)

Anti -Claudin 5 (CLDN5)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified B IH
Tight junctions (TJ) are specialized regions of cell-cell adhesion in epithelial and endothelial cell sheets. Claudins are the major components tight junction strands. In the brain and lung, Claudin 5 was exclusively concentrated at cell-cell borders in endothelial cells of blood vessels, but not at those of epithelial cells. In contrast, Claudin 5 was restricted to endothelial cells of arteries, but not veins and capillaries in the kidney (1). It is also reported that Claudins-2,-3,-4 and -5 showed heterogenic expression and subcellular localization in the rat liver, pancreas and gut.2 Mouse anti-Claudin 5 stained 100% (20/20) of colon cancer tissues with their matched adjacent and remote mucosa. The staining patterns in both the cancer tissues or the matched mucosa are cytoplasmic and membranous. In breast carcinoma, 62% (33/53) of the tissues stained positive, but the staining pattern was mainly cytoplasmic.
Catalog #C5838-47
ApplicationsSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsWestern Blot: 1-3ug/ml. Tested in mouse and rat samples only.
Immunohistochemisty (FFPE): Requires Heat Induced Epitope Retrieval (HIER) using EDTA pH 8.0. Tested in human samples only. Enzyme digestion is not required.
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.
Positive ControlColon Tissue
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS NumberN/A
Clone TypeMonoclonal
IsotypeIgG1
Clone No3G98
HostMouse
SourceMouse
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
PurityPurified
ImmunogenSynthetic peptide corresponding to mouse Claudin 5 protein.
SpecificityRecognizes human Claudin 5 at ~22-24kD. Expected Staining Pattern
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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