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Specifications

Clone Host Grade Applications Monoclonal Mouse Purified B IH

Tight junctions (TJ) are specialized regions of cell-cell adhesion in epithelial and endothelial cell sheets. Claudins are the major components tight junction strands. In the brain and lung, claudin-5 was exclusively concentrated at cell-cell borders in endothelial cells of blood vessels, but not at those of epithelial cells. In contrast, claudin-5 was restricted to endothelial cells of arteries, but not veins and capillaries in the kidney.1 It is also reported that Claudins-2,-3,-4 and -5 showed heterogenic expression and subcellular localization in the rat liver, pancreas and gut.2 Mouse anti-Claudin-5 stained 100% (20/20) of colon cancer tissues with their matched adjacent and remote mucosa. The staining patterns in both the cancer tissues or the matched mucosa are cytoplasmic and membranous. In breast carcinoma, 62% (33/53) of the tissues stained positive, but the staining pattern was mainly cytoplasmic.
Catalog #	C5838-47
Applications	Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions	Immunohistochemisty (FFPE): Requires Heat Induced Epitope Retrieval (HIER) using EDTA pH 8.0. Tested in human samples only. Enzyme digestion is not required.
	Immunohistochemistry: Frozen sections
	Western Blot: 1-3ug/ml. Tested in mouse and rat samples only.
	Optimal dilutions to be determined by the researcher.
Positive Control	Colon Tissue
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG1
Clone No	3G98
Host	Mouse
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Purity	Purified
Immunogen	Synthetic peptide corresponding to mouse claudin-5 protein.
Specificity	Recognizes human Claudin 5 at ~22-24kD. Expected Staining Pattern
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.