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You are here:Home » Antibodies » Abs to Claudin » Anti -Claudin 5 (CLDN5)

Anti -Claudin 5 (CLDN5)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH
Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets. In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell. TJ strands on adjacent cells are presumed to interact with each other to form a sort of "molecular gasket" that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other. In addition to this so-called "barrier" function, the "fence" function of tight junctions plays an important role in maintaining epithelial cell-polarity by blocking the diffusion of membrane proteins between apical (luminal) and basolateral cell surfaces. Confinement of the glucose symport to apical surfaces allows glucose to be transported vectorially from the lumen, through the cell, and into the bloodstream.
Catalog #C5838-47B
ApplicationsSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended DilutionsELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunohistochemistry (Human Colon): 5-10ug/ml
Note: For best results in immunohistochemistry with formalin-fixed, paraffin-embedded (FFPE) tissues, heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining.
Optimal dilutions to be determined by the researcher.
Positive ControlsRat lung, kidney, and small intestine homogenates and mouse lung homogenates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No6A44
HostMouse
SourceRat
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide
PurityPurified by Protein A affinity chromatography
ImmunogenSynthetic peptide corresponding to mouse Claudin-5 protein.
SpecificityRecognizes endogenous levels of rat Claudin-5 protein at ~22-24kD. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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