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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified IH

Claudin 1-8 proteins are a family of transmembrane proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact made up of a network of strands to act as a molecular gasket for preventing the leakage of ions, water etc. between cells. They are abundant in luminal epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different Claudin composition.
Catalog #	C5838-48
Applications	Suitable for use in Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (Formalin/paraffin): 1:12-25 for 30 min
	Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
	Optimal dilutions to be determined by the researcher.
Positive Control	Colon carcinoma.
Cellular Localization	Cell membrane and cytoplasmic.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	N/A
Clone Type	Polyclonal
Host	Rabbit
Source	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide, before the addition of glycerol to 40%.
Purity	Purified by immunoaffinity chromatography.
Immunogen	A synthetic peptide derived from the C-terminus of mouse Claudin 5.
Specificity	Recognizes mouse Claudin 5. Species Crossreactivity: Human
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.