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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP IH

Claudin-7 is one of the transmembrane proteins that seal tight junctions and is critical for maintaining cell-to-cell adhesion in epithelial cell sheets. Claudin-7 is localized in the intercellular junctions of the epithelial cells of the proximal tubules of the nephrons in mammalian kidney. It is reportedly down-regulated in head and neck squamous cell carcinomas and loss of claudin-7 expression has been linked to histological grade in ductal carcinoma and invasive ductal carcinoma. Claudin-7 has been shown to regulate the expression of prostate-specific antigen (PSA) and it is also expressed in normal human and mouse epithelia.
Catalog #	C5838-54B
Applications	Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	ELISA: 0.1-1ug/ml
	Immunoprecipitation (Native): 2ug/IP reaction
	Western Blot: 1ug/ml
	Immunohistochemistry (FFPE): 5ug/ml Requires heat induced epitope retrieval (HIER) with EDTA, pH 8.0 prior to staining.
	Optimal dilutions to be determined by the researcher.
Positive Control	Western Blot: T47D cells
	Immunohistochemistry: Human breast cancer tissues
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	6A45
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography
Immunogen	Synthetic peptide corresponding to the C-terminal region of the human Claudin-7.
Specificity	Recognizes human Claudin-7 at ~22kD. Does not cross-react with Claudin-3 or -4.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.