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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IP IH

Claudin-7 is one of the transmembrane proteins that seal tight junctions and is critical for maintaining cell-to-cell adhesion in epithelial cell sheets. Claudin-7 is localized in the intercellular junctions of the epithelial cells of the proximal tubules of the nephrons in mammalian kidney. It is reportedly down-regulated in head and neck squamous cell carcinomas and loss of claudin-7 expression has been linked to histological grade in ductal carcinoma and invasive ductal carcinoma. Claudin-7 has been shown to regulate the expression of prostate-specific antigen (PSA) and it is also expressed in normal human and mouse epithelia.
Catalog #	C5838-54B
Applications	Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.
Recommended Dilutions	ELISA: 0.1-1ug/ml
	Immunoprecipitation (Native): 2ug/IP reaction
	Western Blot: 1ug/ml
	Immunohistochemistry (FFPE): 5ug/ml Requires heat induced epitope retrieval (HIER) with EDTA, pH 8.0 prior to staining.
	Optimal dilutions to be determined by the researcher.
Positive Control	Western Blot: T47D cells
	Immunohistochemistry: Human breast cancer tissues
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a,k
Clone No	6A45
Host	Mouse
Source	Human
Concentration	~0.5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography
Immunogen	Synthetic peptide corresponding to the C-terminal region of the human Claudin-7.
Specificity	Recognizes human Claudin-7 at ~22kD. Does not cross-react with Claudin-3 or -4.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.