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Specifications

Clone Host Grade Applications Polyclonal Rabbit Purified B IH IF

Claudin-8 is a tight junction protein expressed in several mouse distal nephron segments, suggesting that it may contribute to the paracellular barrier that protects transtubular Na+, K+, and H+ gradients in the distal nephron. Caludin-8 decreases paracellular permeability to cations when overexpressed in MDCK-II cells, suggesting that it may function as a paracellular cation barrier. Caludin-8 is also found in the thin ascending limb of Henle, a segment of the nephron that is known to be leaky to Na+. Claudin-8 is expressed primarily in tight junctions along the entire aldosterone-sensitive distal nephron (ASDN) and in the late thin descending limb of Henle, consistent with its role as a paracellular cation barrier protein. Claudin-8 is found in umbrella cell tight junctions in the bladder epithelium. Expression of Claudin-8 has also been reported in the inner ear.
Catalog #	C5838-56
Applications	Suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Other applications have not been tested.
Recommended Dilution	Immunohistochemistry (frozen): 2.7ug/ml.
	Western Blot: 1-3ug/ml. Detects a band at ~30kD.
	Immunofluorscence: 2.7ug/ml
	Optimal dilutions to be determined by the researcher.
Recommended Controls	TCMK-1, KNRK and MDCK cell lysates
Recommended Tissue	Mouse Colon Tissue
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Concentration	~0.25mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Synthetic peptide derived from the C-terminal region of mouse and rat Claudin-8.
Specificity	Recognizes Claudin-8. Species Crossreactivity: mouse, rat and canine.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.