| Tight junctions act as both the barrier (or gate) and the fence between neighboring cells. The barrier function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces. The fence function maintains cell polarity, thus, tight junctions work as a fence to prevent intermixing of molecules in the apical membrane with |
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| those in the lateral membrane. Tight junctions are comprised of the membrane proteins occludin, claudins, and JAMs. Claudins are responsible for the formation of tight junction strands and are connected with the actin cytoskeleton by ZO-1.1 Claudin-8 is a tight junction protein expressed in several distal nephron segments, suggesting that it may have a role as a |
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| paracellular cation barrier protein.2-4 Claudin-8 is found in the inner ear and the umbrella cell tight junctions in the bladder epithelium |
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| Catalog # | C5838-56A |
| Applications | Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. |
| Recommended Dilution | Immunofluorescence: 8ug/ml |
| Western Blot: 1-3ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Positive Control | T84 colorectal carcinoma cell lysates, HT29/6D, and MDCK-C7 |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | N/A |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.25mg/ml |
| Form | Supplied as a liquid in PBS, 0.1% sodium azide. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Synthetic peptide derived from the C-terminal region of human claudin-8 |
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| Specificity | Recognizes human Claudin-8 protein at ~23kD. Also bands observed at ~15, and ~80kD in . Species Crossreactivity: rat. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
