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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified IC FC

Claudin-12 is a 244 amino acid multipass membrane protein in the claudin family of epithelial tight junction proteins. Claudin-12 is expressed on intestinal, bladder, and blood-brain barrier epithelial cells. Human Claudin-12 shares 92% aa sequence identity with mouse and rat Claudin-12.
Catalog #	C5838-61A
Applications	Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions	Flow Cytometry: 2.5ug/ml labels 1x10e6 cells. Validated for use with CaCo2 human colorectal adenocarcinoma cell line.
	Immunocytochemistry: 8-25ug/ml.
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	7H195
Host	Mouse
Source	Human
Concentration	~0.5mg/ml (after reconstitution)
Form	Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity	Purified by Protein G affinity chromatography.
Immunogen	Recombinant protein corresponding to aa1-244 from human Claudin 12, expressed in HEK293 human embryonic kidney cell line (NP_036261).
Specificity	Recognizes human Claudin 12. Stains human Claudin 12 transfectants but not irrelevant transfectants. Species Sequence Homology
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.