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You are here:Home » Antibodies » Abs to Claudin » Anti -Claudin 15 (CLDN15)

Anti -Claudin 15 (CLDN15)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IF
Tight junctions create circumferential seals around cells in epithelial tissues that are critical for maintaining cell-cell adhesion. The “barrier” function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces, while the “fence” function maintains cell polarity. Tight junctions thus function as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane. Tight junctions are comprised of the membrane proteins occludin, claudins, and JAMs. Claudins are responsible for the formation of tight junction strands and are connected with the actin cytoskeleton by ZO-1.
Claudin-15 is expressed in endothelial cells in the mouse kidney. Claudin-15 creates Na+ -selective paracellular channels, and charge-reversing mutations on the first extracellular domain of Claudin-15 create Cl- -selective paracellular channels. Claudin-15 has a high level of expression in the mouse small and large intestine.
Catalog #C5838-86
ApplicaitonsSuitable for use in Immunohistochemistry, Immunofluorescence and Western Blot. Other applications have not been tested.
Recommended DilutionsImmunohistochemistry (Frozen) (mouse): 14.5ug/ml
Immunofluorescence (mouse): 14.5ug/ml
Western Blot (mouse, human, rat): 1-3ug/ml detects a single band at ~20kD.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.25mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Immunoaffinity chromatography.
ImmunogenSynthetic peptide corresponding to the C-terminal region of mouse Claudin-15.
SpecificityRecognizes mouse Claudin-15. Reactivity has been confirmed with mouse intestine (ileum and colon) homogenates, rat ileum homogenates, and human claudin-15-transfected Xenopus oocyte homogenates. Low levels of expression have also been observed in mouse kidney homogenates by Western Blot.
Species CrossreactivityHuman and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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