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You are here:Home » Antibodies » Abs to Claudin » Anti -Claudin 18 (CLDN18)

Anti -Claudin 18 (CLDN18)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B IH IF
Claudin-18 is a tight junction protein expressed as lung- and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene.1 The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor.1 A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human.1 Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein.1 Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells.1 These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and
stomach.1 Claudin-18 expression has also been reported in the inner ear.2
Catalog #C5838-91
ApplicationsSuitable for use in Western Blot, Immunofluorescence, and Immunohistochemistry Other applications not tested.
Recommended DilutionWestern Blot: 1-3ug/ml
Immunohistochemistry (frozen): 5ug/ml
Immunofluorescence: 5ug/ml
Optimal dilutions to be determined by the researcher.
Positive Control Mouse lung and stomach homogenates
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
Concentration~0.25 mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
PurityPurified by immunoaffinity chromatography
ImmunogenSynthetic peptide derived from the C-terminal region of the human Claudin-18 protein, which differs from mouse by 4 conservative amino acid replacements and one non-conservative amino acid replacement
SpecificityRecognizes mouse Claudin-18 protein at ~29 kD.
Species sequence homologyHuman
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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