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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B IH IF

Claudin-18 is a tight junction protein expressed as lung- and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene.1 The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor.1 A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human.1 Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein.1 Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells.1 These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and
stomach.1 Claudin-18 expression has also been reported in the inner ear.2
Catalog #	C5838-91
Applications	Suitable for use in Western Blot, Immunofluorescence, and Immunohistochemistry Other applications not tested.
Recommended Dilution	Western Blot: 1-3ug/ml
	Immunohistochemistry (frozen): 5ug/ml
	Immunofluorescence: 5ug/ml
	Optimal dilutions to be determined by the researcher.
Positive Control	Mouse lung and stomach homogenates
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Mouse
Concentration	~0.25 mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by immunoaffinity chromatography
Immunogen	Synthetic peptide derived from the C-terminal region of the human Claudin-18 protein, which differs from mouse by 4 conservative amino acid replacements and one non-conservative amino acid replacement
Specificity	Recognizes mouse Claudin-18 protein at ~29 kD.
Species sequence homology	Human
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.