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You are here:Home » Antibodies » Abs to Lectins » Anti -CLEC4E (C-type lectin domain family 4, member E)

Anti -CLEC4E (C-type lectin domain family 4, member E)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IH IF
CLEC4E is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. CLEC4E expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6,6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB). Recently it was reported that CLEC4E is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor gamma-chain (FcR-gamma) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.
Catalog #C5839-66
ApplicationsSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50. Incubated for 2 hours at RT. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).
Immunofluorescence (IF-IC): 1:500
Optimal dilutions to be determined by the researcher.
HybridomaMouse F0 myeloma cells with spleen cells from BALB/c mice.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2b,k
Clone No10K398
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography. ( 95% SDS-PAGE)
ImmunogenRecombinant corresponding to aa41-219 from human CLEC4E expressed in E. coli (NP_055173).
SpecificityRecognizes human CLEC4E.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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