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You are here:Home » Antibodies » Abs to Lectins » Anti -CLEC5A (C-type Lectin Domain Family 5 Member A, C-type Lectin Superfamily Member 5, Myeloid DAP12-associating Lectin 1, MDL-1, CLECSF5, MDL1) (APC)

Anti -CLEC5A (C-type Lectin Domain Family 5 Member A, C-type Lectin Superfamily Member 5,
Myeloid DAP12-associating Lectin 1, MDL-1, CLECSF5, MDL1) (APC)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified FC
CLEC5A is a type II membrane protein belonging to the C-type lectin superfamily. CLEC5A is expressed on monocytes/macrophages, dendritic cells, and NK cells and contributes to cellular activation through its association with DAP12.
Catalog #C5839-75
ApplicationsSuitable for use in Flow Cytometry. Other applications not tested.
Recommended DilutionsFlow Cytometry: 10ul labels up to 1x10e6 cells.
Optimal dilutions to be determined by the researcher.
Positive Control Whole blood monocytes.
Flow Cytometry Protocol1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, add 10ul of C5839-75 to up to 1x10e6 cells and incubate for 30 minutes at RT.
3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer.
4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody.
Storage and StabilityMay be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Freezing APC conjugates will result in a substantial loss of activity. APC is light sensitive.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No11C8
HostMouse
SourceHuman
Concentration~0.01mg/ml
FormSupplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant protein corresponding to the extracellular domain of human CLEC5A expressed in NSO cells (Q9NY25).
SpecificityRecognizes human CLEC5A.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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