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Anti -CLEC5A (C-type Lectin Domain Family 5 Member A, C-type Lectin Superfamily Member 5, Myeloid DAP12-associating Lectin 1, MDL-1, CLECSF5, MDL1) (APC)
Anti -CLEC5A (C-type Lectin Domain Family 5 Member A, C-type Lectin Superfamily Member 5,
Myeloid DAP12-associating Lectin 1, MDL-1, CLECSF5, MDL1) (APC)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
FC |
|
| CLEC5A is a type II membrane protein belonging to the C-type lectin superfamily. CLEC5A is expressed on monocytes/macrophages, dendritic cells, and NK cells and contributes to cellular activation through its association with DAP12. | | | Catalog # | C5839-75 | | Applications | Suitable for use in Flow Cytometry. Other applications not tested. | | Recommended Dilutions | Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated using whole blood monocytes. | | Optimal dilutions to be determined by the researcher. | | Flow Cytometry Protocol | 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. | | 2. After blocking, add 10ul of C5839-75 to 1-2.5x10e5 cells and incubate for 30 minutes at RT. | | 3. Remove unbound antibody by washing the cells twice in Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. | | 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2B antibody. | | Storage and Stability | May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for at 12 months at 4°C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyaninconjugates are sensitive to light. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2b | | Clone No | 11C8 | | Host | Mouse | | Source | Human | | Concentration | Not determined | | Form | Supplied as a liquid in sodium chloride, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC). | | Purity | Purified by Protein G affinity chromatography. | | Immunogen | Recombinant corresponding to the extracellular domain of human CLEC5A. | | Specificity | Recognizes human CLEC5A. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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