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You are here:Home » Antibodies » Antibodies-Membrane Proteins » Anti -CLIMP63 (Cytoskeleton-Linking Membrane Protein of 63kD, ER Protein p63)

Anti -CLIMP63 (Cytoskeleton-Linking Membrane Protein of 63kD, ER Protein p63)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IC FC
The 63kD cytoskeleton-linking membrane protein (CLIMP-63) is a marker for the reticular subdomain of the rough endoplasmatic reticulum (ER) and is excluded from the outer nuclear membrane. Concentration of CLIMP-63 into patches may enhance microtubule binding on the cytosolic side and contribute to ER morphology by the formation of a protein scaffold in the lumen of the ER, linking the rough ER to microtubules.
Catalog #C5840-93
Suitable for use in ELISA, Flow Cytometry, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionsImmunoprecipitation: 2ul
Immunocytochemistry: 1:1000
Immunohistochemistry: Frozen sections
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
Clone No6D315
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography and gel filtrated (FPLC) (> 95%).
ImmunogenHuman CLIMP63 from Caco-2 cells golgi membrane fraction.
SpecificityRecognizes human CLIMP63. Species Crossreactivity: monkey
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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