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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -CLIMP63 (Cytoskeleton-Linking Membrane Protein of 63kD, ER Protein p63)

Anti -CLIMP63 (Cytoskeleton-Linking Membrane Protein of 63kD, ER Protein p63)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B IP IH IC FC
The 63kD cytoskeleton-linking membrane protein (CLIMP-63) is a marker for the reticular subdomain of the rough endoplasmatic reticulum (ER) and is excluded from the outer nuclear membrane. Concentration of CLIMP-63 into patches may enhance microtubule binding on the cytosolic side and contribute to ER morphology by the formation of a protein scaffold in the lumen of the ER, linking the rough ER to microtubules.
Catalog #C5840-93
Applications
Suitable for use in ELISA, Flow Cytometry, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionsImmunoprecipitation: 2ul
Immunocytochemistry: 1:1000
Immunohistochemistry: Frozen sections
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No6D315
HostMouse
SourceHuman
Concentration~1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 0.1% sodium azide.
PurityPurified by Protein G affinity chromatography and gel filtrated (FPLC), 95%.
ImmunogenHuman CLIMP63 from Caco-2 cells golgi membrane fraction.
SpecificityRecognizes human CLIMP63. Species Crossreactivity: monkey
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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