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Anti -CLIMP63 (Cytoskeleton-Linking Membrane Protein of 63kD, ER Protein p63)
Anti -CLIMP63 (Cytoskeleton-Linking Membrane Protein of 63kD, ER Protein p63)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B IP IH IC FC |
|
| The 63kD cytoskeleton-linking membrane protein (CLIMP-63) is a marker for the reticular subdomain of the rough endoplasmatic reticulum (ER) and is excluded from the outer nuclear membrane. Concentration of CLIMP-63 into patches may enhance microtubule binding on the cytosolic side and contribute to ER morphology by the formation of a protein scaffold in the lumen of the ER, linking the rough ER to microtubules. | | | Catalog # | C5840-93 | | Applications | | | Suitable for use in ELISA, Flow Cytometry, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested. | | Recommended Dilutions | Immunoprecipitation: 2ul | | Immunocytochemistry: 1:1000 | | Immunohistochemistry: Frozen sections | | Western Blot: 1:1000 | | Optimal dilutions to be determined by the researcher. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a | | Clone No | 6D315 | | Host | Mouse | | Source | Human | | Concentration | ~1mg/ml (after reconstitution) | | Form | Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 0.1% sodium azide. | | Purity | Purified by Protein G affinity chromatography and gel filtrated (FPLC), 95%. | | Immunogen | Human CLIMP63 from Caco-2 cells golgi membrane fraction. | | Specificity | Recognizes human CLIMP63. Species Crossreactivity: monkey | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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