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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

CLOCK, a basic helix-loop-helix (bHLH)/PAS protein, is an important factor in the molecular clock mechanism. Mutations in this protein lead to abnormal circadian behavior and defective transcriptional activity. CLOCK has been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.
Catalog #	C5843-01
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilutions	Western Blot: 1-10ug/ml using ECL.
	ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide per well.
Control Peptide	C5843-40: Clock, Rabbit (dClock) Control Peptide
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Drosophila
Concentration	~1mg/ml
Form	Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Purity	Purified by affinity chromatography.
Immunogen	A 17aa C-terminus peptide of Drosophila Clock. (KLH coupled)
Specificity	Recognizes Drosophila Clock. The immunogen shows no significant sequence homology with mammalian Clock or Per proteins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.