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You are here:Home » Antibodies » Abs to Circadian Proteins » Anti -Clock

Anti -Clock

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
CLOCK, a basic helix-loop-helix (bHLH)/PAS protein, is an important factor in the molecular clock mechanism. Mutations in this protein lead to abnormal circadian behavior and defective transcriptional activity. CLOCK has been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.
Catalog #C5843-01
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionsWestern Blot: 1-10ug/ml using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide per well.
Control PeptideC5843-40: Clock, Rabbit (dClock) Control Peptide
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceDrosophila
Concentration~1mg/ml
FormSupplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
PurityPurified by affinity chromatography.
ImmunogenA 17aa C-terminus peptide of Drosophila Clock. (KLH coupled)
SpecificityRecognizes Drosophila Clock. The immunogen shows no significant sequence homology with mammalian Clock or Per proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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