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Specifications

Clone Host Grade Applications Polyclonal Sheep Affinity Purified E B

Clock is an essential regulator of circadian rhythms. Clock is a basic helix-loop-helix-PAS transcription factor that heterodimerizes with BMAL1. Together these transcription factors bind to E box elements (CACGTG) within the promoter of circadian genes (period1 and 2) stimulating their transcription. BMAL dimerization is necessary for Clock’s nuclear translocation, E box dependent transcription, phosphorylation, and degradation.
Catalog #	C5843-16
Applications	Suitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended Dilution	Western Blotting: 0.5ug/mL
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	N/A
Clone Type	Polyclonal
Isotype	IgG
Host	Sheep
Source	Human
Concentration	~0.5ug/ml
Form	Supplied as a lyophilized powder in PBS, 0.02% trehalose. Reconstitute with 1ml sterile 40-50% glycerol, PBS.
Purity	Purified by immunoaffinity chromatography.
Immunogen	E. coli-derived recombinant human Clock.
Specificity	Recognizes human Clock.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.