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Specifications

For isolation of Clostridium difficile.
Clostridium Difficile Agar is a formula recommended by George et al. for isolation of C. difficile. The medium contains fructose which enhances the recovery and growth of C. difficile. The addition of the antibiotics, cefoxitin and cycloserine, inhibit the growth of Enterobacteriaceae, Enterococcus faecalis, staphylococci, Gram negative non-sporulating anaerobic bacilli, and Clostridia sp. but not C. difficile. Supplementing the medium with horse blood and egg yolk further improves the recovery of C. difficile.
Catalog #	C5852
Storage	Store the sealed bottle containing the dehydrated medium at 2 to 8.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.
Microbiological Analysis
Bacteroides fragilis ATCC 25285	Inhibited
Clostridium difficile ATCC 9689	Growth
Clostridium perfringens ATCC 13124	Inhibited
Staphylococcus aureus ATCC 25923	Inhibited
Appearance
Light beige, homogeneous, free flowing powder.
Solubility
Light amber,clear to slightly hazy.
pH (25°C)
7.4±0.2
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	Cycloserine-Cefoxitin-Fructose Agar (CCFA)