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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Clostridium difficile is a gram­positive motile bacterium which is the leading cause of diarrhoea in developed countries. The incidence of disease in humans varies greatly with age, spore density and the administration of antibiotics. Toxin B (TcdB) is a large exotoxin. Its role in disease is less well understood than that of toxin A, though it seems clear that TcdB contributes to disease. Both toxins modifies the Ras superfamily of small GTPases via glycosylation, inactivating them and leading to the disruption of vital signaling pathways in the cell.
Catalog #	C5856-01E
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions	ELISA: 1:20-1:200
	Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies	I1904-25D IgG, H&L (AP) Pab Gt xMo
	I1904-67AA IgG, H&L (AP) Pab Gt xMo
	I1904-65N IgG (HRP) Pab Rb xMo
	I1903-07E IgG (HRP) Pab Rb xMo
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	N/A
Clone Type	Monoclonal
Isotype	IgG1
Clone No	9i96
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Specificity	Recognizes Clostridium difficile toxin B. Does not cross-react with other toxins.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.