| The cyclic nucleotides cAMP and cGMP are implicated in signal transduction events such as the visual transduction, relaxation of smooth muscles, intestinal secretion of water and salt, reabsorption of Na+ and water in the distal tubule of the nephrons. cAMP/cGMP activate Ca2+ -permeable ion channels called cyclic nucleotide-gated channels (CNG or CNC). Activation of CNG leads to depolarization of the membrane voltage and to a concomitant increase of the cytosolic Ca2+. CNG consists of two distinct subunits, designated a and b subunits. Several CNG a-subunits (CNG alpha 1-3) & beta subunits (subunit 2 or CNGb1-2 or CNG4-5) and numerous isoforms. a-subunit can form functional channel by themselves, whereas, b-subunits modulate the channel property of a-subunits. CNG display intracellular N and C-termini, 6 transmembrane domains or segments (S1-S6). The region between S5 and S6 contains the ion-conducting pore (P). The cyclic nucleotide-binding region is found at the c-terminus. Native functional CNG may exists as heteromultimer containing some combination of a, and b subunits. |
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| Bovine CNG1 was initially cloned and characterized from rod cells. CNG1 (rat 683 aa, mouse 684, human 686 aa; also describes as OCNC1) is primarily expressed in outer segment of photoreceptor rod cells, and in a variety of tissues (kidney, eye, pineal gland, pituitary, adrenal, spleen, brain, heart, skeletal muscle, and testis). Human/mouse CNG1 exhibit ~88% sequence homology. |
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| Catalog # | C5999-20 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilutions | ELISA: 0.5-1ug/ml Control peptide (C5999-25) can be used to coat ELISA plates at 1ug/ml. |
| Western Blot: 1-10ug/ml using ECL. |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, before the addition of glycerol to 40%. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | A 20aa peptide sequence near C-terminus of Human CNG1. |
| Specificity | Recognizes human CNG1. No significant homology exists with other CNGs. |
| Species sequence homology | Porcine |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
