| The cyclic nucleotides cAMP and cGMP are implicated in signal transduction events such as the visual transduction, relaxation of smooth muscles, intestinal secretion of water and salt, reabsorption of Na+ and water in the distal tubule of the nephrons. cAMP/cGMP activate Ca2+ -permeable ion channels called cyclic nucleotide-gated channels (CNG or CNC). Activation of CNG leads to depolarization of the membrane voltage and to a concomitant increase of the cytosolic Ca2+. CNG consists of two distinct subunits, designated a and b subunits. Several CNG a-subunits (CNG alpha 1-3) & beta subunits (subunit 2 or CNGb1-2 or CNG4-5) and numerous isoforms. a-subunit can form functional channel by themselves. b-subunits modulate the channel property of a-subunits. CNG display intracellular N and C-termini, 6 transmembrane domains or segments (S1-S6). The region between S5 and S6 contains the ion-conducting pore (P). The cyclic nucleotide-binding region is found at the c-terminus. Native functional CNG may exists as heteromultimer containing some combination of a, and b subunits. CNG2 or OCNC1 (rat/mouse/rabbit 664 aa, bovine 663 aa) is primarily expressed in olfactory sensory neurons. It is ~80% homologous with CNG1. Unlike CNG1, CNG2 is activated both by cAMP and cGMP. Another cGMP-gated channel called CNG3 (mouse 537aa, rat 61 aa, human, 694aa, bovine 706aa, chicken 64 aa) has been cloned from heart, kidney, testis, sperm, and taste buds. |
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| Catalog # | C6000-05 |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Western Blot: 1:1000-1:5000 (ECL). |
| ELISA: 1:10,000-1:50,000. Coat ELISA plates at 1ug/ml with Control Peptide. |
| Optimal dilutions to be determined by the researcher. |
| Recommended Control Peptide | C6000-07 |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Rat |
| Concentration | Not determined |
| Form | Supplied as a liquid in PBS, 0.05% sodium azide. |
| Purity | Serum |
| Immunogen | Synthetic peptide corresponding to 18aa sequence near the N-terminus of rat/mouse CNG2 (KLH). Species sequence homology |
| Specificity | Recognizes rat CNG2. No significant sequence homology is detected with other CNGs. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
