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You are here:Home » Antibodies » Abs to Ion Channel » Anti -CNG3 (Cyclic Nucleotide-Gated Channels 3)

Anti -CNG3 (Cyclic Nucleotide-Gated Channels 3)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Serum E B
The cyclic nucleotides cAMP and cGMP are implicated in signal transduction events such as the visual transduction, relaxation of smooth muscles, intestinal secretion of water and salt, reabsorption of Na+ and water in the distal tubule of the nephrons. cAMP/cGMP activate Ca2+ -permeable ion channels called cyclic nucleotide-gated channels (CNG or CNC). Activation of CNG leads to depolarization of the membrane voltage and to a concomitant increase of the cytosolic Ca2+. CNG consists of two distinct subunits, designated a and b subunits. Several CNG a-subunits (CNG alpha 1-3) & beta subunits (subunit 2 or CNGb1-2 or CNG4-5) and numerous isoforms. a-subunit can form functional channel by themselves, whereas, b-subunits modulate the channel property of a-subunits. CNG display intracellular N and C-termini, 6 transmembrane domains or segments (S1-S6). The region between S5 and S6 contains the ion-conducting pore (P). The cyclic nucleotide-binding region is found at the c-terminus. Native functional CNG may exists as heteromultimer containing some combination of a, and b subunits.
Catalog #C6005-03
CNG2 or OCNC1 (rat/mouse/rabbit 664 aa, bovine 663 aa) is primarily expressed in olfactory sensory neurons. It is ~80% homologous with CNG1. Unlike CNG1, CNG2 is actated channel called CNG3 (mouse 537 aa, rat 611 aa, human, 694 aa, bovine 706 aa, chicken 645 aa) has been cloned from heart, kidney, testis, sperm, and taste buds. Deletion of CNG3 gene in mice leads of degeneration of cone photoreceptors.
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 40% glycerol.
PuritySerum
ImmunogenA 14 AA Peptide sequence near N-terminus of rat/mouse CNG3 (1). The peptide was coupled to KLH
SpecificityThe 14 AA rat control peptide is 83% conserved in chicken, 75% in human and 83% in bovine CNG3. No significant sequence homology is detected with other CNGs. The control peptide, because of small size (MW 1.697kD), is not suitable for Western. It should be used for ELISA and antibody blocking to confirm specificity of antibodies
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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