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You are here:Home » Antibodies » Antibodies-Ion Channel » Anti -CNG4 (Cyclic Nucleotide-Gated Channels 4, b-Subunit)

Anti -CNG4 (Cyclic Nucleotide-Gated Channels 4, b-Subunit)


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Clone Host Grade Applications
Polyclonal Rabbit Serum E B
The cyclic nucleotides cAMP and cGMP are implicated in signal transduction events such as the visual transduction, relaxation of smooth muscles, intestinal secretion of water and salt, reabsorption of Na+ and water in the distal tubule of the nephrons. cAMP/cGMP activate Ca2+ -permeable ion channels called cyclic nucleotide-gated channels (CNG or CNC). Activation of CNG leads to depolarization of the membrane voltage and to a concomitant increase of the cytosolic Ca2+. CNG consists of two distinct subunits, designated a and b subunits. Several CNG a-subunits (CNG alpha 1-3) & beta subunits (subunit 2 or CNGb1-2 or CNG4-5) and numerous isoforms. a-subunit can form functional channel by themselves, whereas, b-subunits modulate the channel property of a-subunits. CNG display intracellular N and C-termini, 6 transmembrane domains or segments (S1-S6). The region between S5 and S6 contains the ion-conducting pore (P). The cyclic nucleotide-binding region is found at the c-terminus. Native functional CNG may exists as heteromultimer containing some combination of a, and b subunits.
Catalog #C6010-04
CNG beta-subunit or subunit 2 (also called CNG4) have been cloned from the rod outer segments (hRCNC2a and hRCNC2b) and from olfactory neurons (rOCNC2, also called CNG5). Human RCNC2a (623 aa; missing 1-286 aa) and RCNC2b (909 aa) are alternatively splice and only differ in their N-terminus. The two b proteins show ~30% identity with human a CNG1. Additional alternatively spliced CNG4D (missing 522-530 aa) and CNG4E (missing 515-532 aa) are also expressed. CNG b-subunits form heterooligomeric complex with CNG1-3. The olfactory b-subunit is expressed throughout the nasal epithelium, especially in olfactory sensory neurons, and in the vomeronasal organ. .
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
ConcentrationAs reported
FormSupplied as a liquid, neat serum, 40% glycerol.
ImmunogenA 24 AA Peptide sequence near C-terminus of rat CNG4 (1) was used for antibody production. The peptide was coupled to KLH
SpecificityThe 24 AA rat control peptide is 100% conserved in alternatively spliced rat b-subunits (1339 aa & 858 aa) and 66% in human b-subunits (1251 aa, RCNC2a 623 aa, 1245 b1 and RCNC2b 909 aa). No significant sequence homology is detected with other CNGs. The control peptide, because of small size (MW 2.65kD), is not suitable for Western. It should be used for ELISA and antibody blocking to confirm specificity of antibodies
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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