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Anti -CNG4 (Cyclic Nucleotide-Gated Channels 4, b-Subunit)
Anti -CNG4 (Cyclic Nucleotide-Gated Channels 4, b-Subunit)
Pricing
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| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
E B |
|
| The cyclic nucleotides cAMP and cGMP are implicated in signal transduction events such as the visual transduction, relaxation of smooth muscles, intestinal secretion of water and salt, reabsorption of Na+ and water in the distal tubule of the nephrons. cAMP/cGMP activate Ca2+ -permeable ion channels called cyclic nucleotide-gated channels (CNG or CNC). Activation of CNG leads to depolarization of the membrane voltage and to a concomitant increase of the cytosolic Ca2+. CNG consists of two distinct subunits, designated a and b subunits. Several CNG a-subunits (CNG alpha 1-3) & beta subunits (subunit 2 or CNGb1-2 or CNG4-5) and numerous isoforms. a-subunit can form functional channel by themselves, whereas, b-subunits modulate the channel property of a-subunits. CNG display intracellular N and C-termini, 6 transmembrane domains or segments (S1-S6). The region between S5 and S6 contains the ion-conducting pore (P). The cyclic nucleotide-binding region is found at the c-terminus. Native functional CNG may exists as heteromultimer containing some combination of a, and b subunits. CNG beta-subunit or subunit 2 (also called CNG4) have been cloned from the rod outer segments (hRCNC2a and hRCNC2b) and from olfactory neurons (rOCNC2, also called CNG5). Human RCNC2a (623 aa; missing 1-286 aa) and RCNC2b (909 aa) are alternatively splice and only differ in their N-terminus. The two b proteins show ~30% identity with human a CNG1. Additional alternatively spliced CNG4D (missing 522-530 aa) and CNG4E (missing 515-532 aa) are also expressed. CNG b-subunits form heterooligomeric complex with CNG1-3. The olfactory b-subunit is expressed throughout the nasal epithelium, especially in olfactory sensory neurons, and in the vomeronasal organ. | | | Applications: | | | Suitable for use in ELISA and Western Blot. Other applications not tested. | | | Recommended Dilution: | | | Western Blot: 1-10ug/ml (ECL). | | | ELISA: 0.5-1ug/ml C6010-08 can be used to coat ELISA plates at 1ug/ml. | | | Optimal dilutions to be determined by the researcher. | | | Catalog # | C6010-05 | | Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | | CAS Number | n/a | | Clone Type | Polyclonal | | Isotype | IgG | | Host | Rabbit | | Source | Rat | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4 0.1% BSA. | | Purity | Purified by immunoaffinity chromatography. | | Immunogen | A 24aa synthetic peptide sequence near C-terminus of rat CNG4. (KLH). | | Specificity | Recognizes rat CNG4. No significant sequence homology is detected with other CNGs. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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