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You are here:Home » Antibodies » Abs to Ion Channel » Anti -CNG4 (Cyclic Nucleotide-Gated Channels 4, b-Subunit)

Anti -CNG4 (Cyclic Nucleotide-Gated Channels 4, b-Subunit)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
The cyclic nucleotides cAMP and cGMP are implicated in signal transduction events such as the visual transduction, relaxation of smooth muscles, intestinal secretion of water and salt, reabsorption of Na+ and water in the distal tubule of the nephrons. cAMP/cGMP activate Ca2+ -permeable ion channels called cyclic nucleotide-gated channels (CNG or CNC). Activation of CNG leads to depolarization of the membrane voltage and to a concomitant increase of the cytosolic Ca2+. CNG consists of two distinct subunits, designated a and b subunits. Several CNG a-subunits (CNG alpha 1-3) & beta subunits (subunit 2 or CNGb1-2 or CNG4-5) and numerous isoforms. a-subunit can form functional channel by themselves, whereas, b-subunits modulate the channel property of a-subunits. CNG display intracellular N and C-termini, 6 transmembrane domains or segments (S1-S6). The region between S5 and S6 contains the ion-conducting pore (P). The cyclic nucleotide-binding region is found at the c-terminus. Native functional CNG may exists as heteromultimer containing some combination of a, and b subunits. CNG beta-subunit or subunit 2 (also called CNG4) have been cloned from the rod outer segments (hRCNC2a and hRCNC2b) and from olfactory neurons (rOCNC2, also called CNG5). Human RCNC2a (623 aa; missing 1-286 aa) and RCNC2b (909 aa) are alternatively splice and only differ in their N-terminus. The two b proteins show ~30% identity with human a CNG1. Additional alternatively spliced CNG4D (missing 522-530 aa) and CNG4E (missing 515-532 aa) are also expressed. CNG b-subunits form heterooligomeric complex with CNG1-3. The olfactory b-subunit is expressed throughout the nasal epithelium, especially in olfactory sensory neurons, and in the vomeronasal organ.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 1-10ug/ml (ECL).
ELISA: 0.5-1ug/ml C6010-08 can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Catalog #C6010-05
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceRat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4 0.1% BSA.
PurityPurified by immunoaffinity chromatography.
ImmunogenA 24aa synthetic peptide sequence near C-terminus of rat CNG4. (KLH).
SpecificityRecognizes rat CNG4. No significant sequence homology is detected with other CNGs.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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