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You are here:Home » Antibodies » Abs to Enzymes, Phosphodiesterase » Anti -CNPase (2',3'-cyclic Nucleotide 3'-phosphodiesterase)

Anti -CNPase (2',3'-cyclic Nucleotide 3'-phosphodiesterase)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IH IC
CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligo-dendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively. CNPase has been very useful as a marker for these particular cell types. Developmentally, this enzyme appears early in oligodendrocytes, earlier than most other myelin proteins. It continues to be expressed at high levels in oligodendrocytes and Schwann cells of adult animals. In various diseases, neurological mutants, and in experimental conditions in which myelin is reduced, CNPase levels may be severely reduced. The enzyme itself exists in two forms in most species (three in mouse) with molecular masses of 48 and 46kD on SDS-PAGE, referred to as CNP2 and CNP1, respectively.Studies continue to elucidate the physiological role of the enzyme. CNPase I (46kD) and CNPase II (48kD) are differentially regulated during development, with the larger protein being expressed earlier than CNPase I during development.
Catalog #C6205
Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system. This protein is also recognized by the CNPase (2',3'-cyclic Nucleotide 3'-phosphodiesterase) Mab Mo xHu (Catalog No C6205-01). In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.
ApplicationsSuitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended DilutionsWestern Blot: 1:1,000 using 10ug of mouse brain lysate as sample. Other suitable samples include myelin, the Wolfgram protein fraction, the SN4 fraction, tissue sections
and mixed glial tumors (oligodendrogliomas, etc.)
Immunohistochemistry: Fresh frozen and paraffin embedded tissue. Note: Recommend microwave pretreatment, ctirate pH 6.0 of paraffin sections.
Immunocytochemistry: Primary oligodendrocyte cultures.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No2Q33
HostMouse
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide.
PurityPurified by affinity chromatography.
ImmunogenPurified human brain CNPase (2',3'-cyclic nucleotide 3'-phosphodiesterase) (whloe protein).
SpecificityRecognizes humn CNPase, 48 and 46kD polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc. Species Crossreactivity: bovine, rat, mouse, rabbit, sheep, porcine, canine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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