Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Toxins » Anti -Cytotoxic Necrotizing Factor 1 (CNF1)

Anti -Cytotoxic Necrotizing Factor 1 (CNF1)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B Gel
CNF1 and CNF2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho; however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis
and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter
exhibits the catalytic activity of the toxin.
Catalog #C6400
ApplicationsSuitable for use in Immunoassays, Western Blot, Native dot blot analyses, and for inhibition of biological activity. Other applications not tested.
Recommended DilutionWestern Blot: 1:10
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9G169
HostMouse
SourceE. coli
ConcentrationAs reported
FormSupplied as a liquid in PBS, 0.1% BSA.
PurityPurified
ImmunogenCytotoxic necrotizing factor type 1 (CNF1) of uropathogenic E. coli.
SpecificitySpecific for Cytotoxic necrotizing factor type 1 (CNF1) of uropathogenic Escherichia coli. Recognizes an epitope between aa704 and 730 of the C-terminal enzymatic domain. NG8 specifically neutralizes CNF1 while lacking activity for CNF2.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCNF1


External Links