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You are here:Home » Antibodies » Abs to Toxins » Anti -Cytotoxic Necrotizing Factor 1 & 2 (CNF1, CNF2)

Anti -Cytotoxic Necrotizing Factor 1 & 2 (CNF1, CNF2)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B
CNF1 and 2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho; however, CNF1 and the CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria-related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115kD. CNF1 can be structurally organized into three functional domains the N-terminal binding domain, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin.
Catalog #C6400-01
ApplicationsSuitable for use in Immunoassays, Western Blot, and inhibition of toxin activity of CNF1. JC4 does not inhibit the toxin activity of CNF2. Other applications not tested.
Recommended DilutionWestern Blot: 1:10
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG2a
Clone No9G170
HostMouse
SourceE. coli
Concentration~0.1mg/ml
FormSupplied as a liquid in PBS, 0.1% BSA.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli CNF1/CNF2.
SpecificityRecognizes Cytotoxic necrotizing factor type 1 and the highly related Cytotoxic necrotizing factor type 2 (CNF1 and CNF2) of uropathogenic E. coli. Recognizes an epitope between aa169-191 of the N-terminal binding domain. It neutralizes only CNF1.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCNF1/CNF2


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