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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Cobalt BioAssay™ Kit

Cobalt BioAssay™ Kit

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Specifications

Cobalt is a hard, lustrous, grey metal, a chemical element (symbol Co, atomic number 27 and
atomic mass 58.9). Cobalt is an essential trace-element for all multicellular organisms as the active center of coenzymes called cobalamins. These include vitamin B-12 which is essential for plants and animals [1]. Cobalt is also an active nutrient for bacteria, algae, and fungi, and may be a necessary nutrient for all life. Cobalt, particularly the Co+2 species, forms complexes with a large number of organic and inorganic molecules with a range of extinction coefficients in the visible range. The Cobalt Assay kit takes advantage of the reaction of Co+2 with mercaptoethanol in basic media. Under this condition, Cobalt forms a complex with a strong absorbance band at 475nm. The metal ions Fe+2, Cu+2, Ni+2, Zn+2, Mn+2, exhibit less than 10% interference at this wavelength. The kit provides a quick simple accurate method of quantitating Co+2 in a variety of samples. The assay is linear in the range 3 to 50nmol (0.18-3ug) Cobalt, with a detection limit of ~3nmol (or ~15uM, 0.18ug).
Catalog #C7488-01
Kit ComponentsCobalt Reagent 1x1ml
Cobalt Chloride Standard (1.0umol) lyophilized
Storage and StabilityStore the kit at RT. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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