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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » Coenzyme A BioAssay™ Kit

Coenzyme A BioAssay™ Kit

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Specifications

Coenzyme A (CoA) is composed of units derived from cysteine, pantothenic acid, and ATP. It
plays important roles in the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many other biological processes. One of the main functions of CoA is the carrying and transfer of acyl groups. One of the most important acyl groups transferred is the acetate group, in which case the molecule is called acetyl-CoA. The acetyl group eventually finds itself incorporated into a variety of molecules such as cholesterol, acetylcholine, melatonin, heme and the TCA cycle intermediates. In the assay, free CoA is specifically utilized to generate products which react with OxiRed Probe to generate color (lambda = 570nm) and fluorescence (Ex=535/Em=587nm). The assay can detect 0.1 to 10nmol of CoA (2.5-250uM concentration range) in a variety of samples.
Catalog #C7505-45
Kit Components*CoA Assay Buffer 1x25ml
OxiRed Probe lyophilized
DMSO (anhydrous) 1x0.4ml
Conversion Enzyme Mix lyophilized
CoA Substrate 1x1ml
Acyl CoA Developer lyophilized
CoA Standard (10umol) lyophilized
Storage and StabilityStore the kit at -20°C. Allow *CoA Assay Buffer to warm to RT before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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