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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

Cofilin and ADF (actin-depolymerization factor) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments, by severing preexisting filaments, and the nucleation of new actin polymers (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at serine 3 of cofilin (3-5). Phosphorylation at serine 3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).
Catalog #	C7506-15
Applications	Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution	Western Blot: 1:1000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	As reported
Form	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Purity	Purified by Protein A and peptide affinity chromatography.
Immunogen	Synthetic peptide corresponding to residues surrounding Ser3 of human cofilin (KLH coupled).
Specificity	Recognizes endogenous levels of human total cofilin protein. May recognize the muscle isoform of cofilin 2. Species Crossreactivity
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.