Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Antibodies » Abs to Actin Related Proteins » Anti -Cofilin 1, phosphorylated (Ser3) (Cofilin-1, 18kD Phosphoprotein, p18, Cofilin, Non-muscle Isoform, CFL1, CFL)

Anti -Cofilin 1, phosphorylated (Ser3) (Cofilin-1, 18kD Phosphoprotein, p18, Cofilin,
Non-muscle Isoform, CFL1, CFL)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified B
Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ADF. In humans, cofilin 1 and ADF have 72% identity, with the major aa differences found in the C-terminal region. Regulation of cofilin activity can occur through serine phosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, PP1, PP2A, PP2B, slingshot, and chronophin can dephosphorylate Ser-3 and activate actin binding. Thus, Ser-3 phosphorylation is a major site for the regulation of cofilin activity.
Catalog #C7506-25D
ApplicationsSuitable for use in Western Blot. Other applications not tested.
Recommended DilutionWestern Blot: 1:1000
Optimal dilutions to be determined by the researcher.
Positive ControlJurkat
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide, 50% gylcerol.
PurityPurified by immunoaffinity chromatography.
ImmunogenSynthetic phospho-Synthetic peptide corresponding to aa residues surrounding serine 3 in human Cofilin 1 (KLH).
SpecificityRecognizes human Cofilin 1, phosphorylated at Ser3. Species Crossreactivity: mouse and rat.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links