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You are here:Home » Antibodies » Antibodies-Actin Related Proteins » Anti -Cofilin 2 (CFL2, cofilin 2 (muscle) , HGNC:1875 , cofilin 2)

Anti -Cofilin 2 (CFL2, cofilin 2 (muscle) , HGNC:1875 , cofilin 2)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Catalog #C7506-50B
Alternate Name CFL2, cofilin 2 (muscle) , HGNC:1875 , cofilin 2
Domain Information
Gene Ontology Terms
Application(s)Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 18kD band observed in Human Duodenum, Heart and Muscle lysates (calculated MW of 18.7kD according to NP_068733.1and NP_619579.1). Recommended concentration: 0.01-0.03ug/ml.
0.003ug/ml staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Peptide BlockingCorresponding peptide is available for Peptide Blocking studies. See C7506-50B-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
Clone TypePolyclonal
FormSupplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenC-DIKDRSTLGEK, C Terminus
SpecificityThis antibody is expected to recognize both reported isoforms (NP_068733 and NP_619579).
Species CrossreactivityHuman, mouse, rat, canine, ovine, porcine.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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