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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B

CARF (collaborator of ARF) is a component of the ARF-MDM2-p53 pathway. CARF interacts with ARF in the nucleolus accentuating ARF-mediated p53 stabilization and activation. Data also suggests that in the absence of ARF, CARF interacts directly with p53 in the nucleoplasm resulting in a moderate stabilization and activation of p53.
Catalog #	C7509-70
Applications	Suitable for use in Western Blot and ELISA. Other applications not tested.
Recommended Dilution	Western Blot: 1ug/ml
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Goat
Source	Human
Concentration	As reported
Form	Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, 0.02% sodium azide or sterile 40-50% glycerol.
Purity	Purified by immunoaffinity chromatography.
Immunogen	E. coli-derived recombinant human CARF (aa380-580) GenBank Accession # NM_017632
Specificity	Recognizes human CARF.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.