| This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins. |
|
| Catalog # | C7510-10X |
| Applications | Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. |
| Recommended Dilution | ELISA: 1:5000-1:50,000 |
| Indirect ELISA: 1:4000-1:8000 |
| Western Blot: 1:5000-1:50,000 |
| Immunohistochemistry (frozen): 1:50-1:200 |
| Immunoprecipitation: 1:5000-1:10,000 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.6, 15mg/ml BSA, 0.01% sodium azide. Labeled with Biotin. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Collagen Type I from adult human and bovine tissues. Cellular Localization: Extracellular matrix. |
| Specificity | Recognzies human Collagen Type I. Does not crossreact with Type II, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Species Crossreactivity |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | COL1A1, Collagen Of Skin Tendon And Bone, OI4, Osteogenesis Imperfecta Type IV, Pro alpha 1(I) collagen |
