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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type I (Biotin)

Anti -Collagen Type I (Biotin)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH IF
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
Catalog #C7510-10X
ApplicationsSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended DilutionELISA: 1:5000-1:50,000
Indirect ELISA: 1:4000-1:8000
Western Blot: 1:5000-1:50,000
Immunohistochemistry (frozen): 1:50-1:200
Immunoprecipitation: 1:5000-1:10,000
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.6, 15mg/ml BSA, 0.01% sodium azide. Labeled with Biotin.
PurityPurified by immunoaffinity chromatography.
ImmunogenCollagen Type I from adult human and bovine tissues. Cellular Localization: Extracellular matrix.
SpecificityRecognzies human Collagen Type I. Does not crossreact with Type II, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Species Crossreactivity
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesCOL1A1, Collagen Of Skin Tendon And Bone, OI4, Osteogenesis Imperfecta Type IV, Pro alpha 1(I) collagen


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