| Collagens are highly conserved throughout evolution and are characterized by an uninterrupted “Glycine-X-Y” triplet repeat that is a necessary part of the triple helical structure. It is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on non-denatured three-dimentional epitopes. Collagens from human and bovine placenta and cartilage were extensively purified by limited pepsin digestion and selective salt precipitation. This preparation resulted in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to product “type” specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducting conditions of SDS-PAGE and Immunoblot are used. |
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| Catalog # | C7510-11C |
| Applications | Suitable for use in ELISA, Immunoprecipitation, native PAGE, Immunohistochemistry and Western Blot Other applications not tested. |
| Recommended Dilution | Western Blot: 1:2500-1:25,000 |
| ELISA: 1:2500-1:25,000 (using 1ug of Collagen I) |
| Immunohistochemistry (frozen tissue): 1:25-1:100 |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~0.5mg/ml (UV 280) |
| Form | Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide, before the addition of 40% glycerol. No stabilizing proteins added. |
| Purity | Purified by immunoaffinity chromatography. |
| Immunogen | Collagen Type I from human and bovine placenta |
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| Specificity | Recognizes most mammalian Type I collagens. Demonstrates negligible crossreactivity with Type II, III, IV, V or VI collagens (<1%). Demonstrates negligible non-speci c crossreactivity with anti-collagen antibodies directed against other human serum proteins and non-collagen extracellular matrix proteins. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
