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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type I

Anti -Collagen Type I

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B IP IH
Collagens are highly conserved throughout evolution and are characterized by an uninterrupted “Glycine-X-Y” triplet repeat that is a necessary part of the triple helical structure. It is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on non-denatured three-dimentional epitopes. Collagens from human and bovine placenta and cartilage were extensively purified by limited pepsin digestion and selective salt precipitation. This preparation resulted in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to product “type” specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducting conditions of SDS-PAGE and Immunoblot are used.
Catalog #C7510-11C
ApplicationsSuitable for use in ELISA, Immunoprecipitation, native PAGE, Immunohistochemistry and Western Blot Other applications not tested.
Recommended DilutionWestern Blot: 1:2500-1:25,000
ELISA: 1:2500-1:25,000 (using 1ug of Collagen I)
Immunohistochemistry (frozen tissue): 1:25-1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
Concentration~0.5mg/ml (UV 280)
FormSupplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 0.01% sodium azide, before the addition of 40% glycerol. No stabilizing proteins added.
PurityPurified by immunoaffinity chromatography.
ImmunogenCollagen Type I from human and bovine placenta
SpecificityRecognizes most mammalian Type I collagens. Demonstrates negligible crossreactivity with Type II, III, IV, V or VI collagens (<1%). Demonstrates negligible non-speci c crossreactivity with anti-collagen antibodies directed against other human serum proteins and non-collagen extracellular matrix proteins.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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