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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B IP IH

Catalog #	C7510-11E
Applications	Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution	Western Blot: 1:3000-1:6000
	ELISA: 1:3000-1:6000
	Immunohistochemistry: 1:50-1:200
	Optimal dilutions to be determined by the researcher.
Label	Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Biotin/Protein Ratio	10-20 BAC molecules per rabbit IgG molecule
Storage and Stability	Lyophilized powder may be stored at 4°C Stable for 12 months. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 10mg/ml BSA, 0.01% sodium azide. Labeled with Biotin. Reconstitute with100ul sterile 40-50% glycerol, ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Collagen Type I from human and bovine placenta.
Specificity	Recognizes Collagen Type I. Some class speci c anti-collagens may be speci c for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin- xed, paraf n embedded tissues. Recognizes most mammalian Type I collagens and has negligible crossreactivity with Type II, III, IV, V and VI collagens detected by ELISA against puri ed standards.. Non-speci c crossreaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.