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You are here:Home » Antibodies » Abs to Collagen » Anti -Collagen Type I (Collagen Type 1, Collagen Of Skin Tendon And Bone, Alpha 1 Type I Collagen, Collagen I alpha 1 Polypeptide, Collagen Type I alpha 1, COL1A1, Alpha 2 Type I Collagen, Collagen I alpha 2 Polypeptide, Collagen Type I alpha 2, COL1A2, Osteoge

Anti -Collagen Type I (Collagen Type 1, Collagen Of Skin Tendon And Bone, Alpha 1 Type I
Collagen, Collagen I alpha 1 Polypeptide, Collagen Type I alpha 1, COL1A1, Alpha 2 Type I
Collagen, Collagen I alpha 2 Polypeptide, Collagen Type I alpha 2, COL1A2, Osteoge

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E IH
Catalog #C7510-11K
ApplicationsSuitable for use in ELISA and Immunohistochemistry (frozen and paraffin). Other applications not tested.
Recommended Dilutionimmunohistochemistry (frozen): 1:10-1:50
immunohistochemistry (paraffin): 1:10-1:50
ELISA: 1:500-1:3,000
Requires antigen retrieval using 1mg/ml pepsin in 0.5M acetic acid for 2 hours at 37°C and multiple buffer washes prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C for short-term only.Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceHuman
ConcentrationNot determined
FormSupplied as a lyophilized powder from PBS, 1% dextran, 1% mannitol. Reconstitute with 500ul sterile 40-50% glycerol, ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenCollagen type I from human placenta.
SpecificityRecognizes human Collagen I.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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